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. 2011 Jul 1;121(8):3051–3061. doi: 10.1172/JCI44262

Figure 2. Exogenous RA imprints gut-homing specificity on T cells primed in skin-draining ingLN.

Figure 2

CFSE-labeled OT-I or DO11.10 cells were adoptively transferred into C57BL/6 or Balb/c mice on day 0. 2 hours later, a single dose of Ova was applied orally or injected s.c. A group of s.c. immunized mice received additional s.c. RA injections on days 0, 1, and 2. T cell homing to the small intestine was determined on day 5. (A and C) Representative flow cytometry plots for OT-I (A) and DO11.10 (C) T cell migration to the small intestinal IEL and LPL compartments. (B) Percent Ly5.1+Vβ5+ cells within the gated CD8αβ+ population. (D) Percent DO11.10-TCR+ cells within the gated CD4+ population. Bars represent mean values; symbols represent individual mice pooled from 3 (OT-I) or 2 (DO11.10) independent experiments. *P < 0.05; **P < 0.01.