Table 4.
Evaluation of SNP primer pairs in four carrot genetic stocks used to develop the EST library
| PCR results | Number of primers | Percentage of all primer tested | Percentage of amplified primer | Percentage of amplified and sequenced primers |
|---|---|---|---|---|
| SNP | ||||
| Tested | 354 | - | - | - |
| Amplified | 311 | 88 | - | - |
| Single product | 272 | 77 | 87 | - |
| Single expected product | 162 | 46 | 52 | - |
| Single larger product | 110 | 31 | 35 | - |
| Multiple product | 39 | 11 | 13 | - |
| Sequenced* | 258 | 73 | 83 | - |
| Polymorphic | 212 | 60 | 68 | 82 |
*Considering 162 single expected product and 96 single larger product whit amplicon size <500 nt