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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Mol Immunol. 2011 Oct 26;49(3):423–432. doi: 10.1016/j.molimm.2011.08.026

Figure 4.

Figure 4

IL-6 mRNA stability in cells treated with LPS, pam2CSK4 or LPS plus pam2CSK4. U937 cells were treated with 100ng/ml LPS, 100ng/ml pam2CSK4 or LPS plus pam2CSK4 for 4 hours, followed by addition of 10 μg/ml of actinomycin D. U937 cells were harvested before and 2 and 4 hours after actinomycin D treatment for quantification of IL-6 mRNA using real-time PCR. The IL-6 mRNA expression was normalized to GAPDH mRNA expression and the data presented are % of the control that was IL-6 mRNA level before the addition of actinomycin D.