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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Mol Immunol. 2011 Oct 26;49(3):423–432. doi: 10.1016/j.molimm.2011.08.026

Figure 5.

Figure 5

The Effect of coactivation of TLR4 and TLR2/6 on IL-6 transcription. U937 cells were transiently cotransfected using FuGENE HD transfection reagent with 0.55 μg of p1168huIL6P-luc+ plasmids and pRL-TK plasmids (Renilla luciferase) 24 h. The cells were then treated with 100 ng/ml LPS, 100ng/ml pam2CSK4, or LPS plus pam2CSK4 for 24 h. After the treatment, the cells were lysed and the lysate was used for luciferase activity assay. The firefly luciferase levels were normalized to the Renilla luciferase levels. The presented data (mean ± SD) are representative of 3 experiments with similar results.