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The effect of LPS, pam2CSK4 or both on NFκB DNA-binding activity. U937 cells were treated with 100 ng/ml of LPS, 100 ng/ml of pam2CSK4 or both for 2 or 4 h. At each time point, nuclear protein was extracted and subjected to electrophoretic motility shift assay to determine NFκB DNA-binding activity as described in Methods. The data presented are the representative of 2 independent experiments with similar results.
