Figure 4.

BDNF-induced increase in PSD-95 is abolished by inhibition of MT dynamics. A–D, Images of maximum projection of tubulin and average projection of PSD-95 from time-lapse images, with overlays, before and after BDNF and MT drug treatments. By dampening MT dynamics, MT polymerization into spines (yellow arrows) was almost completely blocked. E, Examples of traces of MT invasions within individual spines. F, Quantification shows no change of PSD-95 level when nocodazole or taxol were included with or without BDNF (Kruskal–Wallis test with Dunn's post hoc tests). Scale bar: (in A) A–D, 2 μm.