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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Mol Immunol. 2011 Oct 19;49(3):495–503. doi: 10.1016/j.molimm.2011.09.024

Figure 7.

Figure 7

Role of CD36 and CD47 in U937-C5aR chemotaxis to C-activated serum versus C-activated plasma. Panel A: Effect of univalent receptor ligation using Fab fragments, Panel B: Effect of peptides to the TSP-1 binding sites. U937-C5aR cells (3×105) in chemotaxis buffer were either not treated (control) or treated for 15 min at 22°C with 25 µg/ml of intact IgG to CD36 or CD47 or 50 µg/ml of the corresponding Fab fragments (panel A) or 100 µg/ml of the CD36 peptides (93–110, 139–155) or the TSP-1 peptide agonist for CD47 (4N1K). Cells were then allowed to respond to either 2.5% C-activated serum or 2.5% C-activated plasma for 120 min at 37°C. Numbers represent mean migration distance ± SEM, n = 4–5. Asterisks denote that cell movement was significantly less (**p < 0.01 or *p < 0.05) compared to the untreated control.