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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Anesth Analg. 2011 Sep 29;113(6):1468–1475. doi: 10.1213/ANE.0b013e31822e913c

Figure 3. Isoflurane-induced elevation of cytosolic calcium levels can be inhibited by 2-DG in H4 naïve cells.

Figure 3

Figure 3

A. The upper panel shows that 10 μM inomycin treatment induces an increase in the fura-2 ratio (340 nm/380 nm) in H4 naïve cells. Treatment with 2-deoxy-d-glucose (2-DG) (lower panel) attenuates the inomycin-induced increase of cytosolic calcium levels in H4 naïve cells. These are representative tracings of two independent experiments. B. Upper panel shows that 2% isoflurane treatment induces a robust increase in the fura-2 ratio (340 nm/380 nm) in H4 naïve cells, which suggests that isoflurane treatment can increase cytosolic calcium levels. Treatment with 2-DG (lower panel) attenuates the isoflurane-induced elevation of cytosolic calcium in H4 naïve cells. These are representative tracings of two independent experiments.