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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Am J Obstet Gynecol. 2011 Jun 24;205(6):576.e7–576.e14. doi: 10.1016/j.ajog.2011.06.064

Figure 1. Effects of HCQ on polyclonal aPL IgG binding and AnxA5 expression on cultured SCTs.

Figure 1

Representative images of three-channel laser confocal microscopy show that in the absence of HCQ, cells treated with polyclonal control IgG showed A) very little bound-IgG (red fluorescence) and B) normal expression of AnxA5 (green fluorescence). Cells treated with polyclonal aPL IgG showed D) a large amount of bound-IgG (red fluorescence) and E) markedly reduced expression of AnxA5 (green fluorescence).

Treatment with 1μg/ml of HCQ G) reduced the amount of bound-aPL IgG and H) increased the expression of AnxA5 on the cells. Addition of HCQ to the polyclonal control IgG-treated cells had no discernible effect (data not shown). C, F and I show merged images of green (FITC), red (rhodamine) and blue (DAPI) fluorescence marking AnxA5, IgG and nuclei, respectively. [bars = 50 μm. Confocal microscopy 3-dimensional projections of Z-axis image stacks, voxel size (μm): width 0.73, height 0.73, and depth 1.50]