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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Am J Obstet Gynecol. 2011 Jun 24;205(6):576.e7–576.e14. doi: 10.1016/j.ajog.2011.06.064

Figure 3. Computer-based quantitative measurements of areas with positive immunofluorescence for AnxA5 and IgG antibodies on the SCTs.

Figure 3

Figure 3

A) In the absence of HCQ, polyclonal aPL IgG significantly reduced the area of AnxA5 with positive immunofluorescence as compared to polyclonal control IgG. In the presence of HCQ, the drug reversed the aPL IgG-mediated reduction of AnxA5 and restored AnxA5 expression on the SCTs.

B) HCQ reversed the binding of aPL IgG to the SCTs. In the absence of HCQ, the cells incubated with polyclonal aPL IgG had a significantly larger area of intense fluorescence for rhodamine-labeled IgG than the cells incubated with polyclonal control IgG. Incubation of the SCTs with aPL IgG together with HCQ markedly reduced the area containing aPL IgG to a level that were much close to the control IgG.