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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Cell Physiol. 2012 Jun;227(6):2470–2479. doi: 10.1002/jcp.22982

Fig. 6. Time course induction of insulin expression from the 3 ITFs/EGF/TGFβ treated AR42J cells.

Fig. 6

The AR42J cells were treated with Ad-LacZ or 3 ITFs with EGF/TGFβ for 3, 7, or 14 days. Cells were harvested from each time point and subjected to (A) RT-PCR, real time PCR analysis, (B) insulin content analysis, (C) and glucose-stimulated insulin secretion assays. RIN cells were used as positive control.