Table 1.
Primer Sequences for RT-PCR
| Gene Name | Forward Primer | Reverse Primer | Size (bps) |
|---|---|---|---|
| Actin | CGTAAAGACCTCTATGCCAA | AGCCAGGGCAGTAATCTCCT | 96 |
| INSM1 | AACTGTCCTTCGCTTGGA | ACGAGACAAACGCGTACAGCT | 80 |
| NeuroD1 | CTTGGCCAAGAACTACATCTGG | GGAGTAGGGATGCACCGGGAA | 228 |
| Pdx-1 | CTCGCTGGGAACGCTGGAACA | GCTTTGGTGGATTTCATCCACGG | 225 |
| Insulin2 | TGGATTCTTCTACACACCCATG | ACGATGCCGCGCTTCTGCC | 276 |
| Nkx6.1 | TACCCCCCATCAAGGATCCATT | GTGCTTCTTTCTCCACTTGGTCC | 223 |
| Nkx2.2 | CCGAGAAAGGTATGGAGGTGAC | CTGGGCGTTGTACTGCATGTGCTG | 187 |
| Pax4 | CTAATGGCTGTGTGAGCAAG | GGGAGAAGATAGTCCGATTC | 386 |
| Pax6 | GGAGTGAATCAGCTTGGTGGT | ATCTGTCTCGGATTTCCCAAGC | 297 |
| Ngn3 | AAGAGCGAGTTGGCACTGAGCAAG | GCTGTGGTCCGCTATGCGCAG | 222 |
| MafA | GCTTCAGCAAGGAGGAGGTCATC | GCCCGCCAACTTCTCGTATTTC | 221 |
| Glut2 | CCAGCACATACGACACCAGACG | CCTGATACGCTTCTTCCAGCAATG | 587 |
| GK | AAGGGAACAACATCGTAGGA | CATTGGCGGTCTTCATAGTA | 130 |
| GHR | CCAACTCCCCTCTACACCAA | GGGCTAACCCTGCCTTAATC | 134 |
| HGFR | CCAGAAGACCAGTTTCCCAA | TCCCATGATAGACACAGCC | 265 |
| IR | GCATCAAACGGACCTATGAT | ACCTGTGTGTTTTCAGGTCC | 281 |
| TGFβR | AACGTTCATGGTTCCGAGAG | ATGTGAAGATGGGCAAGACC | 238 |
| EGFR | AGATGACACATTCCTTCCCG | GTTGTCCAGGCTCATTTGGT | 262 |