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Representative radioligand competition displacement curves for the primary amine NH2-PAT at WT vs. S3.36A and Y7.43A (inset) point-mutated 5-HT2C receptors. Data show means and S.E.M. from an individual experiment of percent specific binding of [3H]-mesulergine. The curve for the S3.36A receptor is significantly (P < 0.05) shifted to the right, whereas the curve for the Y7.43A receptor is not different compared to the WT receptor.
