. Author manuscript; available in PMC: 2012 Dec 30.

Published in final edited form as: Eur J Pharmacol. 2011 Oct 12;673(1-3):1–12. doi: 10.1016/j.ejphar.2011.10.006

Table 3.

EC/IC₅₀ and E/I_MAX values of ligands to activate/inactivate 5-HT_2C receptor-mediated phospholipase C signaling. Functional assays were performed in HEK cells transiently expressing WT, S3.36A or Y7.43A 5-HT_2C receptors. E_MAX/I_MAX values represent percent change from baseline. Data shown are means and S.E.M. (shown in parentheses) of at least 3 independent experiments performed with triplicates. EC/IC₅₀ fold change represents the quantitative fold difference between EC/IC₅₀ values of WT and S3.36A or WT and Y7.43A 5-HT2C receptors, respectively.

LIGAND	WT 5-HT_2C		S3.36A		Y7.43A		FOLD CHANGE
	EC/IC₅₀ (nM)	E/I_max	EC/IC₅₀ (nM)	E/I_max	EC/IC₅₀ (nM)	E/I_max	EC/IC₅₀
Serotonin	5.5 (1.3)	204.7 (11.6)	289.1 (40.4)^a	998.4 (137.1)_b	158.7 (19.9)^a	223.4 (22.7)	52.6, 28.9
N(CH₃)₂- PAT	61.1 (18.2)	163.0 (9.7)	682.5 (102.5)^a	588.0 (62.0) b	519.7 (98.8)^a	180.8 (14.2)	11.2, 8.5
Mesulergine	23.9 (8.3)	82.9 (1.9)	19.9 (8.2)	152.0 (9.2)_b	38.9 (13.3)	79.3 (2.5)	0.8, 1.6

significantly different from WT 5-HT_2C.