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. Author manuscript; available in PMC: 2012 Dec 15.
Published in final edited form as: Free Radic Biol Med. 2011 Sep 16;51(12):2172–2184. doi: 10.1016/j.freeradbiomed.2011.09.005

Fig. 7. Effect of p47phox knockdown on ER stress-induced cell death.

Fig. 7

Effect of p47phox subunit of NADPH oxidase knockdown on thapsigargin challenge (TG, 5 μM, 24 hrs)-induced apoptotic and cell survival responses was assessed. H9C2 myoblasts were transfected with 20 nM siRNA against p47phox or a non-targeting sequence (as control) in DMEM medium for 72 hrs prior to thapsigargin exposure. A: Representative gel blots of p47phox following siRNA knockdown (β-actin was used as the loading control); B: Representative TUNEL assay images in thapsigargin-treated H9C2 myoblasts with or without p47phox knockdown. All nuclei were stained with DAPI shown in blue in the upper panel. The TUNEL-positive nuclei were visualized with fluorescein (green) in the lower panel. Original magnification = 400×; C: Quantified data displaying % of TUNEL positive cells (20 fields); D: Cell survival using MTT assay; and E: Caspase-3 assay; Mean ± SEM, n = 5–7 cultures per group unless otherwise stated, * p < 0.05 vs. control group, # p < 0.05 vs. thapsigargin (TG) group.