Fig. 3.

Localisation of PCR primers for breakpoint sequencing on Mmu17 and Mmu16. a Black lines symbolize the sequence, gray rectangles correspond to the probe array with their location (UCSC NCBI37/mm9/July 2007 assembly), and between brackets is the result of the Cy3/Cy5 ratio in terms of copy number. Full triangles represent the PCR primers. b Electrophoresis of PCR products obtained with different PCR primers on the Mmu17 (primer Up) and Mmu16 (primer Dw). T trisomic DNA; w wild-type DNA. M1 is GeneRuler™ DNA ladder mix (Jena Biosience, Jena, Germany) and M2 is Lambda/HindIII DNA