Fig. 4.

Genomic composition of the Ts65Dn minichromosome. a An exhaustive list of genes located on the Mmu17¹⁶ Ts65Dn according to RefSeq, Ensembl, MGI, and UCSC databases. b Sequence of the Ts65Dn breakpoint. Red and black characters represent Mmu17 and Mmu16 sequences, respectively. The breakpoint is indicated by a slash. Underlined text shows the primer sequences used in Ts65Dn PCR genotyping protocol. c PCR genotyping of the Ts65Dn mice. The forward primer Fw_wt/Ts65Dn is used to amplify both the amplicon specific for the transgenic chromosome (with primer reverse RevTs65Dn) and the wt amplicon, with a reverse primer located on Mmu17 (GGGCAACACTGGATCAATC). After electrophoresis, two bands (396 and 290 bp) are detected by PCR, with DNA isolated from the Ts65Dn animals (Ts) and only one control band (290 bp) from the wild-type animals (wt)