Table 1.
Bacterial strains and plasmids used in this study.
| Strain/plasmid | Genotype or descriptiona | Source |
|---|---|---|
| Strains | ||
| P. aeruginosa | ||
| PAO1 | wild type | T. Köhler |
| ΔpppA-ppkA | PAO1 with unmarked deletion of pppA-ppkA | This work |
| PAO1::tn7TLAC | PAO1::mini-tn7T-LAC | This work |
| Δ::tn7TLAC | ΔpppA-ppkA:: mini-tn7T-LAC | This work |
| Δ::tn7TLACpak | ΔpppA-ppkA:: mini-tn7T-LAC-pppA-ppkA | This work |
| E. coli | ||
| XL1-blue | recA1 endA1 gyrA96 thi hsdR17(rk-, mk+) supE44 relA1 lac [F' proAB+ lacIq Δ(lacZ)M15::Tn10] | Stratagene |
| JM109 | endA1 recA1 gyrA96 thi hsdR17 (rk-mk+) supE44 Δ(lac-proAB), [F' traD36 proAB lacIq Δ(lacZ)M15] | Promega |
| Plasmids | ||
| pEX18Ap | ApR, oriT+ sacB+, gene repacement vector with MSC from pUC18 | (24) |
| pPS858 | ApR, GmR, gfp+, contains GmR-GFP FRT cassette | (24) |
| pFLP2 | ApR, contains FLP recombinase gene | (24) |
| pUC18-mini-Tn7T-LAC | ApR, GmR, mini-Tn7T-Gm Gateway-compatible cloning and delivery vector with lacIq-Ptac expression cassette | (7) |
| pTNS2 | Helper plasmid for integration of the expression casette | (7) |
| pUC18miniTn7TLACpak | pUC18-mini-Tn7T-LAC expressing pppA-ppkA | This work |
aApR, resistant to ampicillin; GmR, resistant to gentamicin