Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Aug 10;11:80. doi: 10.1186/1472-6750-11-80

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright ©2011 Dobosy et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Application of rhPCR to determine genotype for 31 individuals at the SMAD7 rs4939827 SNP Locus. Human DNA samples from 31 individuals comprising 10 C/C homozygotes (circles), 10 T/T homozygotes (squares), and 11 C/T heterozygotes (triangles) were obtained from the Coriell Cell Repository and genotypes were determined using rhPCR. Reactions were run in triplicate using 2 ng of genomic DNA with SYBR^®Green detection. Separate reactions using unmodified non-discriminatory control primers were performed to ensure that the amount of template was approximately equal in each case. A. Reactions were run in real-time mode for 45 cycles. Cq values for triplicate reactions were averaged and plotted according to the primer employed (rC vs. rU). B. Reactions were run in end-point mode for 35 cycles. The raw fluorescence measurements from each reaction are shown and plotted according to the primer employed (rC vs. rU).