Table 2.
Results of phenotypic testing, sequencing, MTBDRsl and spoligotyping
| MDR strains | |||||||
|---|---|---|---|---|---|---|---|
| Number of strains | DST Phenotype | Molecular testing | Spoligotype | ||||
| RMP | FQ | KM/AM | |||||
| rpoB | gyrA | MTBDRsl | rrs (1401, 1402, 1484) | MTBDRsl | |||
| 1 | INH, RMP, SM | S531L | A90V | r | a1401g | r | EAI2-NTB |
| 1 | INH, RMP, SM | D516V | A90V mixed wt | r | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | D94A | r | nd | s | Beijing |
| 2 | INH, RMP, SM | S531L | D94A | r | wt | s | Beijing |
| 1 | INH, RMP, SM | H526Y | D94A | r | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | D94A | r | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | D94A | r | wt | s | Beijing |
| 1 | INH, RMP, SM | L533P | D94A mixed wt | r | wt | s | Beijing |
| 1 | INH, RMP, SM | D516V | D94G | r | wt | s | Beijing |
| 1 | INH, RMP, SM | H526D | D94G | r | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | D94G | r | wt | s | EAI1_SOM |
| 1 | INH, RMP, SM | S531L | D94G | r | wt | s | U |
| 1 | INH, RMP, SM | S531L | D94G mixed wt | r | wt | s | U |
| 1 | INH, RMP | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | H526N L533P | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | H526R | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | R529Q mixed wt | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | D516G S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | D516V | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | D516V | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | D516V | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | D516V | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | D516V | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | D516V mixed wt | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | D516V mixed wt; S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | insertion ttc 514; A532V | wt | s | nd | s | Beijing |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 2 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | wt | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | S512N T525T H526P L527Q | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | H526D | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | H526Y | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | H526Y | wt | s | wt | s | Beijing |
| 2 | INH, RMP, SM | H526N L533P | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | H526R | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | H526R | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | L533P | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | D516V mixed wt; S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | insertion ttc 514 | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | insertion ttc 514 | wt | s | wt | s | Beijing |
| 2 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 2 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | L524W T525P del 526 | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | wt | wt | s | wt | s | Beijing |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Beijing-like |
| 1 | INH, RMP, SM | wt | wt | s | wt | s | Beijing-like |
| 1 | INH, RMP | del 518 | wt | s | wt | s | EAI1_SOM |
| 1 | INH, RMP | S531L | wt | s | wt | s | EAI1_SOM |
| 1 | INH, RMP | wt | wt | s | nd | s | EAI1_SOM |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | EAI1_SOM |
| 1 | INH, RMP, SM | H526D | wt | s | wt | s | EAI1_SOM |
| 1 | INH, RMP, SM | S531L | wt | s | nd | s | EAI1_SOM-EAI2 |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | EAI4_VNM |
| 1 | INH, RMP, SM | H526D | wt | s | wt | s | EAI5 |
| 2 | INH, RMP, SM | D516Q Q517N del 518 | wt | s | wt | s | EAI5 |
| 1 | INH, RMP, SM | S531L | wt | s | nd | s | EAI5 |
| 1 | INH, RMP | L533P | wt | s | wt | s | EAI5 |
| 1 | INH, RMP | S531L | wt | s | wt | s | EAI5 |
| 1 | INH, RMP | L533P | wt | s | wt | s | U |
| 1 | INH, RMP | D516V | wt | s | wt | s | U |
| 1 | INH, RMP, SM | D516V mixed wt | wt | s | wt | s | U |
| 1 | INH, RMP, SM | H526D | wt | s | wt | s | U |
| 2 | INH, RMP, SM | H526D | wt | s | wt | s | U |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | U |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | U |
| 1 | INH, RMP | H526D mixed wt | wt | s | wt | s | Unknown |
| 1 | INH, RMP | S531L | wt | s | wt | s | Unknown |
| 1 | INH, RMP | S531L | wt | s | wt | s | Unknown |
| 1 | INH, RMP | wt | wt | s | wt | s | Unknown |
| 1 | INH, RMP | wt | wt | s | wt | s | Unknown |
| 1 | INH, RMP, SM | H526Y | wt | s | wt | s | Unknown |
| 1 | INH, RMP, SM | H526D R529Q | wt | s | wt | s | Unknown |
| 2 | INH, RMP, SM | S531L | wt | s | wt | s | Unknown |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Unknown |
| 1 | INH, RMP, SM | H526Y | wt | s | wt | s | Unknown |
| 1 | INH, RMP, SM | D516V | wt | s | wt | s | Unknown |
| 1 | INH, RMP, SM | D516V | wt | s | nd | s | Unknown |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Unknown |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Unknown |
| 1 | INH, RMP, SM | S531L | wt | s | wt | s | Unknown |
| 1 | INH, RMP, SM | D516G | wt | s | nd | s | ZERO |
INH isoniazid, RMP rifampicin, SM streptomycin
AM amikacin, FQ fluoroquinolones, KM kanamycin
wt wild type, s susceptible, r resistant, nd not determined