Table 3.
Putative EPS genes in H.somni 2336 and 129Pt with proposed roles in polysaccharide synthesis
| Gene | ORF (HSM-H. somni 2336 and HS- H. somni 129Pt) |
Protein annotation | No. of amino acids, predicted mass (kDa) | % Similarity to another protein |
|---|---|---|---|---|
| galU | HSM_1063 HS_1117 |
UTP-glucose-1-phosphate uridylyltransferase | 295, 32.2 | 70, to glucose-1-phosphate uridylyltransferase, galU (E. coli) |
| manB | HSM_1062 HS_1118 |
Phosphomannomutase | 454, 50.3 | 81, to phosphomannomutase, cpsG (E. coli) |
| csrA | HSM_1061 HS_1119 |
Carbon storage regulator | 60, 6.75 | 89, to pleiotropic regulatory protein for carbon source metabolism, csrA (E. coli) |
| pldB | HSM_1242 HS_0775 |
Lysophospholipase | 318, 37.4 | 49, to lysophospholipase L2, pldB (E. coli) |
| ybhA | HSM_1241 HS_0774 |
Haloacid dehalogenase-like hydrolase | 273, 30.8 | 60, to phosphatase//phospho transferase, ybhA (E. coli) |
| araD | HSM_1240 HS_0773 |
L-ribulose-5-phosphate 4-epimerase | 231, 25.8 | 82, to L-ribulose-5-phosphate 4-epimerase, yiaS (E. coli) |
| sgbU | HSM_1239 HS_0772 |
Putative L-xylulose-5-phosphate 3-epimerase | 290, 33.2 | 84, to L-xylulose 5-phosphate 3-epimerase, yiaQ (E. coli) |
| rmpA | HSM_1238 HS_0771 |
3-keto-L-gulonate-6-phosphate decarboxylase | 215, 23.6 | 64, to 3-keto-L-gulonate 6-phosphate decarboxylase, yiaQ (E. coli) |
| xylB | HSM_1237 HS_0770 |
L-xylulose kinase | 484, 53.7 | 75, to L-xylulose kinase, lyxK (E. coli) |
| rbs1C | HSM_1236 HS_0769 |
Ribose ABC transporter, permease | 342, 32.9 | 59, to D-ribose transporter subunit, rbsc (E. coli) |
| rbs1A | HSM_1235 HS_0768 |
Ribose ABC transporter, ATPase component | 496, 56.1 | 60, to D-ribose transporter subunit, ATP-binding component, rbsA (E. coli K12) |
| rbs1B | HSM_1234 HS_0767 |
ABC-type sugar transport system, periplasmic component | 312, 31.0 | 56, to D-ribose transporter subunit, periplasmic component (E. coli ) |
| glsS | HSM_1233 HS_0766 |
Gluconolaconase | 295, 32.6 | 46, to gluconolactonase, gnl (Zymomonas mobilis) |
| rbs2B | HSM_1232 HS_0765 |
ABC-type sugar-binding periplasmic protein | 369, 37.2 | 81, to hypothetical protein (Yersinia intermedia ATCC 29909) |
| rbs2C | HSM_1231 HS_0764 |
Ribose ABC transporter, permease | 349, 36.9 | 90, to inner-membrane translocator (Yersinia intermedia ATCC 29909) |
| rbs2A | HSM_1230 HS_0763 |
Ribose ABC transporter, ATPase component | 505, 55.8 | 81, to ABC transporter-related protein (Yersinia intermedia ATCC 29909) |
| dctP | HSM_1229 HS_0762 |
TRAP C4-dicarboxylate transport system, periplasmic component | 328, 33.4 | 52, to C4-dicarboxylate binding protein, periplasmic component, dctP (Rhodobacter capsulatus) |
| dctM | HSM_1228 HS_0761 |
TRAP C4-dicarboxylate transport system, permease component | 426, 43.2 | 59, to C4-dicarboxylate -binding protein, permease component, dctM (Rhodobacter capsulatus) |
| dctQ | HSM_1227 HS_0760 |
Tripartite ATP-independent periplasmic transporter | 160, 17.8 | 40, to tripartite ATP-independent periplasmic transporter, dctQ (Rhodobacter capsulatus) |