Figure 1.

Northern and Southern analyses of RINO1. A, Total RNA (10 μg per lane) isolated from unorganized calli (Uc), embryogenic calli (Ec), and organogenetic calli (Oc) was separated on agarose gels containing formaldehyde, blotted, and hybridized with a ³²P-labeled pRINO1 insert. Positions and sizes (in kb) of rRNAs are indicated. B, Total RNA isolated from roots (Rt) and shoots (Sh) 7 d after imbibition, from flowers (F), and from zygotic embryos 7 DAA (Em) were hybridized with the RINO1 probe as in A. C, Rice genomic DNA (5 μg per lane) digested with EcoRI (E), HindIII (H), or XhoI (X) were separated on an agarose gel, blotted, and hybridized with the RINO1 probe. Positions and sizes (in kb) of markers are indicated.