Figure 5.
In situ localization of the RINO1 transcript detected by a DIG-labeled antisense probe (A–E) and globoids detected by toluidine blue staining (F–J) in the aleurone layer in the dorsal side of seeds 3 (A and F), 5 (B and G), 7 (C and H), 10 (D and I), and 14 DAA (E and J). Arrows indicate the border between the aleurone layer (bottom) and maternal tissues (top). en, Endosperm. Some of the globoids in G are marked with arrowheads. Scale bars represent 50 μm.