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. 2011 Jul 14;10:192. doi: 10.1186/1475-2875-10-192

Figure 1.

Figure 1

Construction of recombinant expression vectors. Expression vectors for wheat germ (pIVEX1.4) and E. coli (pIVEX2.4) were originally purchased from Roche. GST was subsequently introduced into these vectors between the 6xHis tag and the multiple cloning site (MCS) to generate plasmids pIVEGST1.4 and pIVEXGST2.4. Amino acids between GST and P. vivax proteins are the same for all constructs.