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Immunohistochemical staining for CA IX (a, e, g), HIF-1α (b, f, h), GLUT1 (c) and VEGF (d) in WTs. Co-localisation of CA IX, HIF-1α, GLUT1 and VEGF in perinecrotic blastema (a-d). Perinecrotic tubules show CA IX but not HIF-1α expression (e, f). Perinecrotic blastemal cells are negative for CA IX, but nuclear expression of HIF-1α is present (g, h).
