Figure 2.

Western blot of seedling extracts from the wild-type (WT) and GS-overexpressing (GS2, GS7, and GS10) transgenic Indian mustard seedlings (digitized image). Equal amounts (10 μg) of total protein were loaded onto each lane, separated on a 10% SDS-PAGE gel, blotted, and treated with antiserum raised against purified E. coli GS protein. Samples were pooled from 25 seedlings each and grown on one-half-strength Murashige and Skoog medium. E. coli extract was included as a positive control.