Figure 3.

Effect of buffer concentration on GA₃ activation of carboxypeptidase III (2437 cDNA) and thiol protease (2529 cDNA) transcript accumulation at neutral pH. De-embryonated half grains were incubated for 0, 1, 2, or 5 d on filter paper soaked with sodium phosphate buffer (NaPhos) at the indicated concentration and pH 7.0, supplemented with 10 mm CaCl₂ and 5 μm GA₃. RNA samples (10 μg) were loaded, electrophoresed, blotted onto Hybond-N filters, and hybridized with ³²P-labeled 2529 cDNA (thiol protease) or 2437 cDNA (carboxypeptidase III). Even loading was checked by rehybridizing filters with ³²P-labeled 18S rRNA.