Table 1.
Information on the primers used for real time PCR
| Gene | Accession # | Primers | Product Size (bp) | Efficiency Rate (E) | |
|---|---|---|---|---|---|
| PPIA | NM_017101 | F | GTCAACCCCACCGTGTTCTTC | 133 | 1.93 |
| R | ATCCTTTCTCCCCAGTGCTCAG | ||||
| TBP | NM_001004198 | F | ACCGTGAATCTTGGCTGTAAAC | 123 | 2.06 |
| R | CGCAGTTGTTCGTGGCTCTC | ||||
| RPII | AB017711 | F | GGCTCTCCAGATTGCGATGTG | 124 | 1.93 |
| R | CAGGTAACGGCGAATGATGATG | ||||
| β-actin | NM_031144 | F | CAGGGTGTGATGGTGGGTATGG | 115 | 2.03 |
| R | AGTTGGTGACAATGCCGTGTTC | ||||
| GAPDH | NM_017008 | F | CAAGTTCAACGGCACAGTCAAG | 123 | 1.91 |
| R | ACATACTCAGCACCAGCATCAC | ||||
| 18s | X01117 | F | CACGGGTGACGGGGAATCAG | 105 | 2.03 |
| R | CGGGTCGGGAGTGGGTAATTTG | ||||
| TASK-1 | NM_033376 | F | GCAGAAGCCGCAGGAGTTG | 126 | 2 |
| R | GCCCGCACAGTTGGAGATTTAG | ||||
| TASK-2 | AM229406 | F | ACGCCCTCTACCGCTACTTTG | 129 | 2.12 |
| R | GCCGCCTCCTCCTCTTCTTG | ||||
| TASK-3 | NM_053405 | F | CGGTGCCTTCCTCAATCTTGTG | 144 | 1.8 |
| R | TGGTGCCTCTTGCGACTCTG |
Nucleotide sequences for the primers, size of PCR products, and PCR amplification efficiency rate of each primer set. All the genes are from rat origin. The real-time PCR efficiency rate (E) in exponential phase was calculated according to the equation: E = 10[-1/slope] [32].