Table 2.
Ranking of the 6 selected reference genes in rat whole carotid bodies by geNorm, NormFinder and BestKeeper
| geNorm | N1+N14 (n = 16) | N14+Hyper14 (n = 14) | N14+Hypo14 (n = 14) | N1+N14+Hyper14+ Hypo14 (n = 28) | ||||
|---|---|---|---|---|---|---|---|---|
| Rank | Gene | M-value | Gene | M-value | Gene | M-value | Gene | M-value |
| Best two genes | RPII TBP | 0.26 | PPIA TBP | 0.20 | PPIA TBP | 0.29 | PPIA TBP | 0.26 |
| 3 | PPIA | 0.27 | RPII | 0.35 | RPII | 0.36 | RPII | 0.38 |
| 4 | actin | 0.34 | GAPDH | 0.43 | GAPDH | 0.45 | GAPDH | 0.44 |
| 5 | GAPDH | 0.38 | actin | 0.50 | actin | 0.50 | actin | 0.49 |
| 6 | 18s | 0.40 | 18s | 0.57 | 18s | 0.52 | 18s | 0.53 |
| Norm Finder | N1+N14 (n = 16) | N14+Hyper14 (n = 14) | N14+Hypo14 (n = 14) | N1+ N14+Hyper14+ Hypo14 (n = 28) | ||||
| Rank | Gene | Variability | Gene | Variability | Gene | Variability | Gene | Variability |
| Best two genes | TBP Actin | 0.009 | RPII GAPDH | 0.007 | RPII GAPDH | 0.008 | PPIA TBP | 0.011 |
| 1 | GAPDH | 0.01 | RPII | 0.013 | TBP | 0.007 | TBP | 0.015 |
| 2 | PPIA | 0.011 | GAPDH | 0.013 | PPIA | 0.015 | GAPDH | 0.017 |
| 3 | actin | 0.014 | TBP | 0.014 | GAPDH | 0.018 | 18s | 0.019 |
| 4 | TBP | 0.015 | actin | 0.019 | RPII | 0.018 | PPIA | 0.020 |
| 5 | RPII | 0.016 | PPIA | 0.021 | 18s | 0.020 | RPII | 0.021 |
| 6 | 18s | 0.018 | 18s | 0.021 | actin | 0.021 | actin | 0.023 |
| Best Keeper | N1+N14 (n = 16) | N14+ yper14 (n = 14) | N14+Hypo14 (n = 14) | N1 + N14 + Hyper14+ Hypo14 (n = 28) | ||||
| Rank | Gene | Coeff. of corr.[r] | Gene | Coeff. of corr.[r] | Gene | Coeff. of corr.[r] | Gene | Coeff. of corr.[r] |
| 1 | PPIA | 0.89 | RPII | 0.97 | RPII | 0.86 | PPIA | 0.97 |
| 2 | actin | 0.84 | PPIA | 0.96 | PPIA | 0.85 | TBP | 0.95 |
| 3 | GAPDH | 0.83 | GAPDH | 0.96 | TBP | 0.85 | GAPDH | 0.95 |
| 4 | 18s | 0.73 | TBP | 0.95 | actin | 0.75 | RPII | 0.93 |
| 5 | RPII | 0.72 | actin | 0.93 | GAPDH | 0.69 | actin | 0.92 |
| 6 | TBP | 0.70 | 18s | 0.87 | 18s | 0.56 | 18s | 0.89 |
The reference genes are ranked in several groups of CB as follows: N1+N14 = immature and mature CB, reared in normoxia (n = 16). N14+Hyper14 = mature normoxia and hyperoxia treated CB (n = 14). N14+Hypo14 = mature normoxia and hypoxia treated CB (n = 14). N1+N14+Hyper14+Hypo14 = immature, normoxia (N1), mature, normoxia (N14), mature, hyperoxia (Hyper14), and mature, hypoxia treated (Hypo14) CB (n = 28). Genes are ranked on their stability value calculated by each respective program: geNorm, M-value; NormFinder, Variability; and BestKeeper, Coefficient of correlation [r]. (n) = number of independent determinations.