Ability of PMC to inhibit the patatinolytic
activity of extracts from 26-month-old tubers. A, SDS-PAGE of purified
PMC (black arrow) and patatin (white arrow) (3.5 and 7.6 μg per lane,
respectively) isolated from 6-month-old tubers. B, Patatin (white
arrow) was added to extracts from 26-month-old tubers, which were then
incubated for 0, 15 and 30 min (37°C) in the absence or presence of
PMC (black arrow). A total of 100 μg of protein containing 48 μg of
patatin and 12 μg of PMC (where indicated) at time zero was loaded in
each lane.