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Absorption spectra and immunoblots depicting the effects of tuber age on protein oxidation. Carbonyl groups of oxidized proteins were derivitized with DNPH and quantified at A380. Oxidized proteins in the soluble-protein fraction of 6-, 18-, and 30-month-old tubers over 20 d of sprouting are shown in the western blot, where protein-carbonyl-DNP derivatives were probed with monoclonal anti-DNP antibody.
