Fig. 4.

a Rho inhibitor CT04 was pretreated for 2 h before UDP stimulation at the indicated concentrations. After 3 min of UDP stimulation, the cells were lysed and subjected to Western blot analysis. CT04 suppressed VASP phosphorylation in a dose-dependent manner. b The cells were pretreated with CT04 (0.5 μg/ml) for 2 h and then stimulated with UDP. After 3 min of UDP stimulation, the cells were fixed and stained with phalloidin (red) and DAPI (blue). UDP-induced actin aggregation (indicated by arrow heads) was decreased by CT04 pretreatment. c The lentivirus expressing p115-RGS or the mutant form of p115-RGS (mut-RGS) were added to the mixed cell culture and incubated for 1 week. Microglial cells were isolated and stimulated by UDP for 3 min. Expression of p115-RGS in microglia, but not mutant form of p115-RGS, suppressed UDP-induced VASP phosphorylation