Table 1.

Biological activity and response of the studied compounds at HEK293 L9-2 cells transiently transfected with hGPR17 using the GloSensor cAMP assay in comparison with [³⁵S]GTPγS binding assay

^aResponse is expressed as percentage of the maximal relative luminescence units and is referenced against UDP, set at 100%

^bConcentration of agonist required to produce 50% of the maximum effect

^cConcentration of antagonists that produces 50% inhibition of agonist effect. Each concentration was tested five times in triplicate, and values are given as the mean ± SE

^dData taken from [³⁵S]GTPγS binding assay [6, 11]