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Roscovitine inhibits NHEJ activity synergistically when combined with Doxorubicin treatment in A549 cells. A) Analysis by real time PCR of NHEJ plasmid re-ligation activity of untreated A549 cell lysate with the addition of 20 μM Roscovitine, DMSO or Wortmannin. B) Analysis by real time PCR of NHEJ plasmid re-ligation activity in A549 cells treated for 12 hours with 20 μM Roscovitine. Wortmannin was added to untreated cell lysate as a negative control for NHEJ activity in vitro.
