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. 2011 Nov 9;11:68. doi: 10.1186/1471-213X-11-68

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Copyright ©2011 Kawakami et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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PDGF-AA Soaked Bead Implantation Affected the NCC Migration. Fluorescent imaging of the cells expressing EGFP, which shows the migration pattern of NCCs in an E9.5 P0-Cre/CAG-CAT-EGFP embryo after the implantation of a control bead (A) or of a PDGF-AA soaked bead (B) in the cranial region. Each figure is an individual frame from a time-lapse movie. The time interval between frames is indicated between the figures. Three differently colored curved arrows (red, yellow, and blue) represent examples of tracing for three distinct migrating NCCs. White circles with dotted lines indicate the position of the implanted bead (n = 5 in each bead implantation).