Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2012 Sep 23.

Published in final edited form as: Biochem Biophys Res Commun. 2011 Aug 27;413(2):299–305. doi: 10.1016/j.bbrc.2011.08.091

Fig. 3 — Acacetin inhibited HIF-1α expression by affecting its degradation. (A) OVCAR-3 and A2780 cells were treated with acacetin (0, 5, 10, and 20 μM) for 6 h. Total RNAs were extracted, and HIF-1α level was assayed using semi-quantitative RT-PCR. (B) Acacetin increased HIF-1α protein degradation. OVCAR-3 and A2780 cells were pretreated with solvent or 40 μM of acacetin for 30 min, followed by addition of CHX (100 μM). The cells were then harvested at different times as indicated. HIF-1α expression was detected by immunoblotting with β-actin as an internal control. (C) Levels of HIF-1α protein were determined by measuring the density of HIF-1α protein band, and normalized to that of β-actin. The relative HIF-1α protein level at time zero was defined as 100%. The experiments were performed 3 times. * indicates significant difference compared to the treatment of CHX alone (P<0.05).

Fig. 3 — Acacetin inhibited HIF-1α expression by affecting its degradation. (A) OVCAR-3 and A2780 cells were treated with acacetin (0, 5, 10, and 20 μM) for 6 h. Total RNAs were extracted, and HIF-1α level was assayed using semi-quantitative RT-PCR. (B) Acacetin increased HIF-1α protein degradation. OVCAR-3 and A2780 cells were pretreated with solvent or 40 μM of acacetin for 30 min, followed by addition of CHX (100 μM). The cells were then harvested at different times as indicated. HIF-1α expression was detected by immunoblotting with β-actin as an internal control. (C) Levels of HIF-1α protein were determined by measuring the density of HIF-1α protein band, and normalized to that of β-actin. The relative HIF-1α protein level at time zero was defined as 100%. The experiments were performed 3 times. * indicates significant difference compared to the treatment of CHX alone (P<0.05).