Figure 5.
Long-term effects of diet schedule on motor activity and anxiety-like behavior in female Wistar rats. Panels show (M ± S.E.M.) (A) 12-h diurnal motor activity, represented as percent of Chow/Chow beam breaks. Rats were tested on Days 1, 4 (C phase) and 6 (P phase) of experimental Week 10 (n = 6–8/group), (B) elevated plus-maze behavior (left) percent of total arm time directed towards the open arms (lower % open arm time signifies more anxiogenic-like behavior), and (right) number of closed arm entries, an index of locomotor activity. Rats were tested during Week 8, 5–9 h after switches from preferred diet (B → A phase) or from chow diet (A → B phase), in a between-subjects design (n = 8–16/group), or (C) time spent in the withdrawal chamber during the defensive withdrawal test. Rats were tested during Week 8, 5–10 h after switches from palatable diet to chow diet (B → A phase) (n = 5–16/group). In all studies, the phase-appropriate diet and water were available ad libitum. Symbols indicate significant differences (p < 0.05) from *Chow/Chow; #Chow/Preferred Day 1 motor activity.