FIGURE 3.
Multiplex cytokine profiling of FL-DCs. A, The FL-DCs were cultured with various doses of virus (25, 50, and 100 ng) and analyzed for their ability to produce different cytokines. LPS was used as positive control. B, Kinetics of select cytokines. The cytokines (IL-6, IL-10, IL-12, IL-23, IFN-γ, and TNF-α) observed in A were further analyzed for their kinetics and were found to respond in a dose- (25, 50, and 100 ng virus) and time-dependent (0, 2, 4, 8, 12, and 24 h) manner. Statistical significance was determined by the Student t test. **p < 0.05; ***p < 0.001.