Figure 2. Polymerization of varied tau concentrations measured by thioflavin S staining.
Wild-type (closed circles •) and 7-Phos tau isoforms (open squares □) polymerization reactions with 0 to 4 μM protein (x-axis) and 75 μM arachidonic acid were measured by thioflavin S fluorescence (y-axis) after 18 hours incubation at 25 °C. Individual graphs are shown for (A) 0N3R, (B) 1N3R, (C) 2N3R, (D) 0N4R, (E) 1N4R, and (F) 2N4R isoforms. Bars represent the mean from 3 experiments ± standard deviation.