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. 2011 Nov 17;17:2986–2996.

Table 1. Primer sequences.

ID Name Sequence
Primer for RT–PCR/RT-qPCR
p1f
FRMD7-FL-f
5′-CAAAGCAGGTAAAAAATCCTAAGG-3′
p1r
FRMD7-FL-r
5′-ATGTGAGATACCATCAACGCTGT-3′
p2f
FRMD7-S-f
5′-TAACAAAGCAGGTAAAAATGGAC-3′
p2r
FRMD7-S-r
5′-AAGATGTGAGATACCATCAACG-3′
p3f
GAPDH-f
5′- CCCACTCCTCCACCTTTGAC-3′
p3r
GAPDH-r
5′- CATACCAGGAAATGAGCTTGACAA-3′
Primer for cloning
pAf
pcDNA3.1-FRMD7-FL-HA-f
5′-CGCTAGCGCCACCATGCTACATTTAAAAGTGCAGT-3′
pAr
pcDNA3.1-FRMD7-FL-HA-r
5′-GCTCGAGTTAAGCGTAATCTGGAACATCGTATGGGTAAGCTAAAAAGTAATTACATG-3′
pBf
pcDNA3.1-FRMD7-S-Myc-f
5′-CGCTAGCGCCACCATGCTACATTTAAAAGTGCAGT-3′
pBr
pcDNA3.1-FRMD7-S-Myc-r
5′-GCTCGAGTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCAGCTAAAAAGTAATTACATG-3′
pCf
pEGFP-FRMD7-FL-f
5′-CGCTAGCATGATTCCCAGAAGAT-3′
pCr
pEGFP-FRMD7-FL-r
5′-GCTCGAGAGCTAAAAAGTAATTACA-3′
pDf
pDsRed-FRMD7-S-f
5′-CGCTAGCATGATTCCCAGAAGAT-3′
pDr pDsRed-FRMD7-S-r 5′-GCTCGAGAGCTAAAAAGTAATTACA-3′

Underlined nucleotides mark the HA-tag and Myc-tag.