Table 1. Primer sequences.
ID | Name | Sequence |
---|---|---|
Primer for RT–PCR/RT-qPCR | ||
p1f |
FRMD7-FL-f |
5′-CAAAGCAGGTAAAAAATCCTAAGG-3′ |
p1r |
FRMD7-FL-r |
5′-ATGTGAGATACCATCAACGCTGT-3′ |
p2f |
FRMD7-S-f |
5′-TAACAAAGCAGGTAAAAATGGAC-3′ |
p2r |
FRMD7-S-r |
5′-AAGATGTGAGATACCATCAACG-3′ |
p3f |
GAPDH-f |
5′- CCCACTCCTCCACCTTTGAC-3′ |
p3r |
GAPDH-r |
5′- CATACCAGGAAATGAGCTTGACAA-3′ |
Primer for cloning | ||
pAf |
pcDNA3.1-FRMD7-FL-HA-f |
5′-CGCTAGCGCCACCATGCTACATTTAAAAGTGCAGT-3′ |
pAr |
pcDNA3.1-FRMD7-FL-HA-r |
5′-GCTCGAGTTAAGCGTAATCTGGAACATCGTATGGGTAAGCTAAAAAGTAATTACATG-3′ |
pBf |
pcDNA3.1-FRMD7-S-Myc-f |
5′-CGCTAGCGCCACCATGCTACATTTAAAAGTGCAGT-3′ |
pBr |
pcDNA3.1-FRMD7-S-Myc-r |
5′-GCTCGAGTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCAGCTAAAAAGTAATTACATG-3′ |
pCf |
pEGFP-FRMD7-FL-f |
5′-CGCTAGCATGATTCCCAGAAGAT-3′ |
pCr |
pEGFP-FRMD7-FL-r |
5′-GCTCGAGAGCTAAAAAGTAATTACA-3′ |
pDf |
pDsRed-FRMD7-S-f |
5′-CGCTAGCATGATTCCCAGAAGAT-3′ |
pDr | pDsRed-FRMD7-S-r | 5′-GCTCGAGAGCTAAAAAGTAATTACA-3′ |
Underlined nucleotides mark the HA-tag and Myc-tag.