Figure 3.

Effect of 60 mmHg hydrostatic pressure on ZO-1 in TM cells after 24 h. ZO-1 was immunolabeled with FITC and the nucleus was immunolabeled with hoechst, and observed under a confocal microscope, using identical parameters. The ZO-1 distribution in iHTM is clearly and regular, while that in GTM₃ is irregular and tangled. Cells under 60 mmHg hydrostatic pressure showed significant decreased and irregular distribution of ZO-1 in both iHTM and GTM₃ . The blank group showed that PBS was used instead of mouse antibody against ZO-1, other steps are the same. Every picture involved in the figure is the Z-depth slide that contain most green fluorescence (scale bar=10 µm).