Figure 7.

Culture of limbal epithelial cells cultivated on electrospun PCL nanofibers by explant method. A: Confluent limbal epithelial cell sheet grown on PCL nanofibers with round and ovoid morphology examined using phase contrast microscopy at day 14 (100× magnification). B: SEM image, LECs’ growth initiated from the edge of the explant (black arrowhead). C: SEM image showing that epithelial cells are closely attached to each other with tightly opposed cell junctions, and D: apical surface showing numerous short microvilli.