Figure 9. Suppression of bAP-evoked Ca²⁺ changes is insensitive to many pharmacological agents.

A, typical experiment. APs were evoked at 1 Hz and the [Ca²⁺]_i increases were measured at a location in the dendrites. After the third AP a UV flash evoked a Ca²⁺ wave near that location and the amplitude of the bAP signals was suppressed for more than 10 s. The experiment was repeated in the presence of 5 μm staurosporine with no clear change in the profile of the Ca²⁺ signals. The amplitude of the Ca²⁺ wave in staurosporine (and in other drugs included in this figure) was within 10% of the amplitude in control conditions. B, the normalized peak amplitude was plotted for the both trials. For quantitative measurements amplitudes were determined 12 s after the wave when the residual amplitude recovered to baseline levels (Fig. 4B). Combined results of experiments on 5 cells showing that the bAP signal amplitude was unaffected by staurosporine at any time. C, summary histogram of experiments testing 7 conditions.