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mNoc mRNA is expressed in multiple tissues of the C3H/He mouse. Tissues for RNA extraction were collected at Zeitgeber Time (ZT) 12 (time of normal light offset) except for that from embryo and thymus, which were purchased from Ambion (Austin, TX); the latter samples were from Swiss mice. Ten μg of total RNA was loaded in each lane except for liver where only 5 μg was loaded. The lower panel shows methylene blue staining of the 28 and 18s ribosomal RNA bands after blotting.
