Figure 7.

mRNA expression of purinergic receptors in PC12N cells and spinal cord. PC12N cells were incubated without (-) or with (+) 50 ng/ml NGF for 5 days. Total RNA was extracted from the cells and the spinal cord and subjected to RT-PCR using the primers shown in Table 1. PCR products were analyzed by 2% agarose gel electrophoresis and stained with ethidium bromide. β-Actin and G3PDH were used for PC12N cells and for the spinal cord as the control and total RNA without RT was used as negative control.