Table 2.

Partition, permeability and diffusion coefficients of vitamin B12 and lysozyme as a function of β-sheet crystallinity of silk films.

Treatment	β Sheet (%)	Compound	Partition Coefficient (Kd)	Permeability Coefficient (cm/s) (× 10−1)	Diffusion Coefficient (D) (cm2/s) (× 10−6)
6 h W.A.	23	B12	4.76 ± 1.77	1.08 ± 0.064*	6.38 ± 0.37*
15 h W.A.	24		5.35 ± 1.42	1.55 ± 0.213*	7.34 ± 1.02†
15 h W.A. + 2 h M.A.	28		6.45 ± 0.39	0.41 ± 0.104*	1.64 ± 0.28*†‡
2 h M.A.	36		12.51 ± 1.04*	0.16 ± 0.017*	0.34 ± 0.03*†‡
6 h W.A.	23	Lysozyme	72.91 ± 4.55*†	0.029 ± 0.003†*	0.056 ± 0.011*
15 h W.A.	24		29.23 ± 5.24*	0.059 ± 0.022	0.057 ± 0.021
15 h W.A. + 2 h MA.	28		21.48 ± 4.88†‡	0.086 ± 0.025†	0.113 ± 0.032*†
2 h M.A.	36		51.14 ± 13.40‡	0.077 ± 0.033*	0.042 ± 0.018†

^{‡, †, *}Data statistically significant (p < 0.05), n=3

W.A. – Water annealed, M.A. – Methanol annealed