Fig. 3.

Effect of Tyk2 deficiency on TGTP expression levels.

Macrophages were treated with poly(I:C) for the times indicated or left untreated (untr., 0 h) and whole cell lysates were analyzed by (A) 2D-DIGE and (B) Western blotting or (C) total RNA was isolated and mRNA expression determined by RT-qPCR. (A) Expression levels as determined by 2D-DIGE (untr. and 18 h poly(I:C)) are given as fold ratios relative to untreated WT cells. Mean values ± SD of three biological replicates are depicted. (B) 10 μg protein per lane was separated by SDS-PAGE (10%T) and probed with an anti-TGTP antibody (short and long exposures are depicted). ERK p42/p44 was used as loading control. Data are representatives of three independent experiments. (C) Mean values of TGTP mRNA expression ± SE from three independent experiments are depicted. Expression levels are shown relative to untreated WT cells, Ube2d2 was used for normalization. Significance levels of genotype-specific differences for each time point are indicated with * p ≤ 0.05 and ** p ≤ 0.01. Significance levels not indicated in the figures are for WT untreated vs. all treatment time points p ≤ 0.01, Tyk2^−/− untreated vs. all treatment time points p ≤ 0.01.