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1H NMR of imino proton resonances as a function of temperature. (A) 7-deaza-dA DDD-1 duplex. (B) Unmodified DDD duplex. Modified and unmodified duplexes were prepared at 0.3 mM and 1.8 mM concentration respectively. The samples were prepared in 10 mM NaH2PO4, 0.1 M NaCl, and 50 μM Na2EDTA at pH 7.0.
