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. 2011 Apr 8;22(11):1389–1401. doi: 10.1089/hum.2011.031

FIG. 5.

FIG. 5.

Detection of AAV8 capsid protein or GFP protein in the germinal centers of macaque spleens. (ac) Spleen and liver from animal 605067 (pre-NAb titer, 1:10; harvested 35 days after receiving AAV8 vector). Immunostaining shows the presence of capsid protein in germinal centers of the spleen (a). GFP is undetectable in the spleen (shown on the same spleen section) (b) or in the liver (c). (df) Spleen and liver from animal 605103 (pre-NAb titer <1:5, harvested on day 35 after vector administration). No capsid protein is detectable in the spleen by immunostaining (d). The germinal centers contain GFP (e) and GFP expression is visible in the liver (f). (gi) Double immunofluorescence staining of spleen from animal 605067 with antibodies directed against follicular dendritic cells (FDCs) (g) and AAV8 capsid (h), showing colocalization (i) within germinal centers. (jl) Double staining of FDCs and capsid shown at higher magnification. Spleen images have been counterstained with DAPI (shown in blue) to visualize nuclei.