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. Author manuscript; available in PMC: 2012 Oct 3.
Published in final edited form as: FEBS Lett. 2011 Aug 16;585(19):2986–2991. doi: 10.1016/j.febslet.2011.08.010

Figure 3.

Figure 3

Isoleucine editing activities of AN2690-resistant and charged Asp487 mutants of LeuRS. Deacylation reactions included approximately 2 mg/ml [14C]-Ile-tRNAleu and 0.5 μM enzyme. Symbols are: wild type (■), D487G (▲), D487N (▼), D487A (◆), D487K (□), D487R (◇), D487E (△) and no enzyme (x). Error bars for each time point are a result of each reaction repeated in triplicate.